Single-Stranded RNA Phages – TheBacteriophags.org

Single-Stranded RNA Phages

by Jan van Duin and Nina Tsareva

Department of Biochemistry
Gorlaeus Laboratories, Leiden University
P.O. Box 9502, 2300 RA Leiden, The Netherlands

Fig. 15-1:	Genetic maps of four representative ssRNA phages. (tif)
Fig. 15-2:	Virus representations… (tif)
Fig. 15-2a:	…Schematic representation of a Levivirus. (tif)
Fig. 15-2b:	…E. coli bacterium with F-pili to which many MS2 phages have attached. (tif)
Fig. 15-3:	RNA secondary structure in the central part of MS2 RNA. (tif)
Fig. 15-4:	Structure in the 5' UTR of MS2 RNA. (tif)
Fig. 15-5:	RNA structure at the coat gene start in Q_{β and MS2.} (tif)
Fig. 15-6:	Localisation of major Q_β polymerase (R) and host factor (H) binding sites in the M site region (RD1) of Q_β RNA. (tif)
Fig. 15-7:	Collection of pseudorevertants obtained after passaging Q_β mutated in its 3' TD through wt or HF deficient host. (tif)
Fig. 15-8:	Evolution of the stabilized coat-protein initiator-hairpin. (tif)
Fig. 15-9:	Evolutionary reconstruction of the deleted coat-protein initiator-hairpin in MS2 RNA... (tif)
Fig. 15-9a:	...at the sequence level. (tif)
Fig. 15-9b:	…at the level of secondary structure. (tif)
Fig. 15-10:	Evolution of MS2 mutant AL20 containing multiple point mutations in the replicase operator hairpin. (tif)
Fig. 15-11:	Acceptance or rejection of various RNA structure elements introduced in a non-coding region of MS2. (tif)
Fig. 15-12:	Comparison of RNA secondary structures in the 3' UTR in four representative RNA phages. (tif)
Fig. 15-13:	Proposed phylogenetic tree for the single-stranded RNA phages. (tif)
Bibliography:	References cited by chapter (pdf file).