Figure 43-3: The key features of pET vectors. In each diagram the relevant elements of T7 are located within the solid segment. All pET vectors are based on the promoter of gene 10 of phage T7 (pØ10) (89). Some include the rbs (S10) of this gene and those shown include the transcription terminator T#216; downstream of gene 10. In the simple translation vector the NdeI site permits fusion of a coding sequence to the rbs of gene 10, while insertion at the BamHI site generates a fusion protein. More recent vectors include a series of targets for alternative restriction enzymes – a multiple cloning site (mcs). (for better image quality, click here for tif image, which may be quite large; may also require screen refreshing plus special software to view; or view larger tif in separate window)

Figure 43-1, Figure 43-2, Figure 43-3, Figure 43-4

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